Validation of the interaction between miRNAs and mRNAs. A) Schematic representation of the standard reporter constructs used for the experimental validation of miRNA-mRNA interactions. WT, MUT and PM denote appropriate constructs; a construct bearing a wild-type potential binding site for the studied miRNA (WT), a construct with mutated binding site (MUT), and a construct with full complementarity (PM). B). Regulation of the ataxin-3 transcript by miR-9-2 and miR-181a-1. Relative repression of luciferase expression for both miRNAs is shown. The standard errors were calculated from independent experiments. The asterisk indicates statistical significance (p value < 0.05).