Figure 2From: Current understanding of the role of microRNAs in spinocerebellar ataxias Validation of the interaction between miRNAs and mRNAs. A) Schematic representation of the standard reporter constructs used for the experimental validation of miRNA-mRNA interactions. WT, MUT and PM denote appropriate constructs; a construct bearing a wild-type potential binding site for the studied miRNA (WT), a construct with mutated binding site (MUT), and a construct with full complementarity (PM). B). Regulation of the ataxin-3 transcript by miR-9-2 and miR-181a-1. Relative repression of luciferase expression for both miRNAs is shown. The standard errors were calculated from independent experiments. The asterisk indicates statistical significance (p value < 0.05).Back to article page