Figure 2
From: Coeliac disease patients do not produce antibodies to a common cerebellar epitope
CD patient serum has a heterogeneous immunoreactivity to mouse brain protein extracts. Western blots of mouse brain protein extracts probed for cross-reacting antibodies with CD and control patient serum. Mouse cerebellar (a) and cerebral cortex (b) total protein extracts were probed with CD and control serum for IgG cross-reacting antibodies. Both CD and control serum identified numerous bands of varying size and intensity. The circled bands indicate immunoreactive proteins that are unique to the cerebellum, and identified by CD but not control serum. Bands indicated by parenthesis have markedly less intensity or cerebral cortex Western. CD4 and 5 are the identical twins. TG6 rabbit polyclonal did not detect either the long 79 kDa or short 71 kDa isoform on the two brain regions tested. However, the TG6 antibody detected a band of 170 kDa in the cerebellum and bands of 30–32, 60 and >260 kDa in cerebral cortex extracts. T = anti-TG6 antibody and SG = rabbit anti-gliadin antibody.