Figure 3
From: Coeliac disease patients do not produce antibodies to a common cerebellar epitope
CD patient serum binds to human cerebellar and cerebral cortex protein extracts in a heterogeneous manner. Western blots of human brain protein extracts were probed for cross-reacting antibodies with CD and control patient serum. Normal human cerebellar (a) and cerebral cortex extracts (b) were probed with patient serum for cross-reacting IgG antibodies. CD and control patient serum bound to numerous bands with varying intensity. The circled bands indicate immunoreactive proteins that are unique to the cerebellum immunoreactive to CD but not control serum. Non-identical twins, CD4 and CD5, have immunoreactive patterns different from each other. The arrow heads at ~52 kDa in both panels indicate IgG heavy chain detected by the secondary rabbit anti-human IgG Ab. The rabbit TG6 antibody only detected a >260 kDa band in the cerebral cortex (b). T = anti-TG6 antibody.