Figure 4
From: Coeliac disease patients do not produce antibodies to a common cerebellar epitope
Mouse cerebellar sections were immunostained with control and CD patient serum for cross-reacting IgG antibodies. Calbindin staining (a) was used as a control to identify PC somata and dendrites. Anti-Hu human auto-immune serum positive control (b) demonstrates nuclear immunoreactivity of PCs and granular cells. (Light- blue nuclei indicate co-localisation with DAPI). Anti-Yo auto-immune positive control (c) immunostained the cytoplasm of PCs in a granular pattern. Typical control patient immunostaining pattern is demonstrated by serum from C1 (d) and C5 (e) showing non-specific immunostaining of PCs and non-specific granular layer staining. Representative images of typical CD patient serum immunostaining PCs are shown with CD4 (f) and CD11 (g). CD11 (g) also demonstrated immunoreactivity to a subset of interneurons. Serum from patient CD10 (h) had unique immunoreactivity to all cerebellar nucleoli as well as the cytoplasm of PCs. Arrows indicate PCs. Asterisk indicates interneurons. Scale bar =100 μm.