Fig. 3
From: Inferior Olivary nucleus degeneration does not lessen tremor in essential tremor
Degenerative changes in the patient’s dentate nucleus (b, d, f, h) compared with a control (a, c, e, g) with LH&E stain (a-d), GAD immunostain (e-f) and calbindinD28k immunostain (g-h). a, b Myelin pallor in the patient’s amiculum (a) and hilum (h) of the dentate nucleus. c, d Dentate neuronal density is normal, although the soma of these neurons was often atrophic (d). Marked reduction of afferent synaptic density from Purkinje cells in dentate demonstrated by GAD (e, f) and calbinidinD28k immunostain (g, h). GAD + −synaptic puncta around large GAD-negative dentate neurons (e, carets in bottom panel) are reduced (f), and some residual synaptic puncta are enlarged (F, small black arrow in bottom panel). GAD + −dentate neurons (e, f, black arrows) are readily identified. Degeneration and/or sprouting of residual Purkinje cell axons is evident (h, small black arrows and inset). There is artifactual staining of atrophic dentate neurons (h). Scale bars: 500 μm, A, B; 100 μm, C, D, and E, F (top panel), G, H. 50 μm, E, F (bottom panel), H, inset